Variations and coding features of the sequence spanning the replication terminus of Bacillus subtilis 168 and W23 chromosomes

Abstract

In a comparative study of the sequences of the 3-kb regions of DNA spanning the replication terminus, terC, of Bacillus subtilis strains 168 and W23, it was found that the latter contained an insertion of a large open reading frame (ORF405) whose translated protein product is a member of the cytochrome P-450 family. The insertion was about 34 nucleotides upstream from a putative promoter for the rtp gene. The sequenced regions contained a number of other ORFs. The translation product of one (ORF238) is a member of a previously identified oxidoreductase superfamily. The translation product of another (ORF257) is significantly similar to the proC product of Escherichia coli, but this ORF does not code for a functional proC product of B. subtilis.