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. 1991 May 1;88(9):4001-4.
doi: 10.1073/pnas.88.9.4001.

Construction and characterization of a single-chain catalytic antibody

R A Gibbs  1 , B A Posner, D R Filpula, S W Dodd, M A Finkelman, T K Lee, M Wroble, M Whitlow, S J Benkovic
Affiliations

Construction and characterization of a single-chain catalytic antibody

R A Gibbs et al. Proc Natl Acad Sci U S A. .

Abstract

The antigen-binding (Fab) fragment of the catalytic monoclonal antibody NPN43C9 has recently been cloned by using bacteriophage lambda. By inserting the variable regions of this Fab coding sequence into a (NH2)-VL-linker-VH-(COOH) construct (where VL and VH represent the heavy and light chain variable regions), we have assembled a recombinant gene encoding a catalytic single-chain antigen-binding protein. This protein has been expressed in Escherichia coli and exhibits the same catalytic parameters as the parent monoclonal antibody NPN43C9. Single-chain forms of catalytic antibodies may prove valuable for structural and site-directed mutagenesis studies as well as for large-scale applications of catalytic antibodies.

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References

    1. J Biol Chem. 1980 Jan 10;255(1):27-9 - PubMed
    1. J Mol Biol. 1980 Jun 15;140(1):57-75 - PubMed
    1. Gene. 1985;33(1):103-19 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Aug;85(16):5879-83 - PubMed
    1. Science. 1988 Sep 2;241(4870):1188-91 - PubMed

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