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. 1992 Aug;30(8):2173-6.
doi: 10.1128/jcm.30.8.2173-2176.1992.

Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction

M Altamirano  1 , M T Kelly, A Wong, E T Bessuille, W A Black, J A Smith
Affiliations

Characterization of a DNA probe for detection of Mycobacterium tuberculosis complex in clinical samples by polymerase chain reaction

M Altamirano et al. J Clin Microbiol. 1992 Aug.

Abstract

We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.

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