Sequence of a putative glutathione synthetase II gene and flanking regions from Anaplasma centrale

Abstract

The complete nucleotide sequence of a putative glutathione synthetase gene (gsh II) has been determined from Anaplasma centrale. The predicted 308 amino acid protein has a molecular weight of 34,222 and is 32% identical to the enzyme, glutathione synthetase (EC 6.3.2.3), encoded by Escherichia coli gsh II. The previously proposed ATP-binding site is not highly conserved. The putative glutathione synthetase gene (gsh II) is preceded by an unassigned open reading frame. Downstream of gsh II is the 5' region of an open reading frame encoding a protein with significant similarity to bacterial D-alanine:D-alanine ligases (ADP forming) (EC 6.3.2.4). The predicted partial amino acid sequence is 33% identical to the amino acid sequence of the protein encoded by the E. coli ddl gene.