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. 1992 Apr;58(4):1402-7.
doi: 10.1128/aem.58.4.1402-1407.1992.

Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52

Y Tan  1 , K J Miller
Affiliations

Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52

Y Tan et al. Appl Environ Microbiol. 1992 Apr.

Abstract

In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50,241. The predicted enzyme was found to contain an amino acid sequence highly homologous to the putative substrate-binding domain present within all lipases examined to date.

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