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Comparative Study
. 1992 Apr 15;89(8):3175-9.
doi: 10.1073/pnas.89.8.3175.

Human combinatorial antibody libraries to hepatitis B surface antigen

S L Zebedee  1 , C F Barbas 3rd, Y L Hom, R H Caothien, R Graff, J DeGraw, J Pyati, R LaPolla, D R Burton, R A Lerner, et al.
Affiliations
Comparative Study

Human combinatorial antibody libraries to hepatitis B surface antigen

S L Zebedee et al. Proc Natl Acad Sci U S A. .

Abstract

Human antibody Fab fragments that bind to hepatitis B surface antigen (HBsAg) were generated by using a recombinant phage surface-display expression system. Characterization of HBsAg-specific Fab fragments isolated from two vaccinated individuals reveals diversity in specificity of antigen binding and in the sequences of the complementarity-determining region. The sequence results show examples of human light-chain promiscuity that result in fine specificity changes and a strong relationship to a human germ-line gene. This application illustrates further that this technique is a powerful tool to isolate distinct human antibodies against immunogenic viral targets.

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