Rearrangement of viral sequences in cytopathogenic pestiviruses
- PMID: 1329326
- PMCID: PMC7131167
- DOI: 10.1016/0042-6822(92)90199-y
Rearrangement of viral sequences in cytopathogenic pestiviruses
Abstract
Two cytopathogenic isolates of bovine viral diarrhea virus (cpBVDV) have been analyzed. For both viruses two regions of their genomic RNAs were found to be duplicated and rearranged. The viral genomes contain a small duplicated element (SD) derived from the genomic 5' end far downstream of its original context. This sequence is followed by a larger duplication which encompasses the region coding for the protein p80(LD), a molecular marker for cpBVDV. The SD element codes for the viral protease p20. In the case of the viruses analyzed here the aminoterminus of p80 is generated by autoproteolytic removal of the preceding SD-encoded protease. For one of the cpBVDV isolates a specific fusion protein (p28) could be identified which is composed of p20 and part of p10, another viral protein. Molecular characterization of the respective noncytopathogenic counterpart revealed that duplication and rearrangement of sequences as well as the expression of p28 and p80 are specific for the cytopathogenic virus.
References
-
- Baker J.C. Bovine viral diarrhea virus: A review. J. Amer. Vet. Med. Assoc. 1987;190:1449–1458. - PubMed
-
- Beaucage S.L., Caruthers M.H. Deoxynucleoside phosphoramidites: A new class of key intermediates for deoxynucleotide synthesis. Tetrahedron Lett. 1981;22:1859–1862.
-
- Bolin S.R., McClurkin A.W., Cutup R.C., Coria M.F. Severe clinical disease induced in cattle persistently infected with noncytopathic bovine viral diarrhea virus by superinfection with cytopathic bovine viral diarrhea virus. Amer. J. Vet. Res. 1985;46:573–576. - PubMed
-
- Brownlie J., Clarke M.C., Howard C.J. Experimental production of fatal mucosal disease in cattle. Vet. Rec. 1984;114:535–536. - PubMed
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials