Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 May;59(5):1683-7.
doi: 10.1128/aem.59.5.1683-1687.1993.

Screening by polymerase chain reaction of Bacillus thuringiensis serotypes for the presence of cryV-like insecticidal protein genes and characterization of a cryV gene cloned from B. thuringiensis subsp. kurstaki

A P Gleave  1 , R Williams, R J Hedges
Affiliations

Screening by polymerase chain reaction of Bacillus thuringiensis serotypes for the presence of cryV-like insecticidal protein genes and characterization of a cryV gene cloned from B. thuringiensis subsp. kurstaki

A P Gleave et al. Appl Environ Microbiol. 1993 May.

Abstract

Polymerase chain reaction screening using cryV-specific oligonucleotides, designed to amplify the 5' half of cryV-type genes, revealed the presence of such genes in 7 of 21 Bacillus thuringiensis serotypes examined. Restriction analysis and hybridization studies indicated that these putative genes fall into at least three subclasses. The nucleotide sequence of the cryV-type gene cloned from B. thuringiensis subsp. kurstaki DSIR732 revealed an open reading frame coding for a protein of 719 amino acids, and lysates of Escherichia coli cells expressing the 81.2-kDa CryV732 protein were toxic to Epiphyas postvittana (Lepidoptera: Tortricidae).

PubMed Disclaimer

References

    1. Mol Microbiol. 1992 May;6(9):1211-7 - PubMed
    1. J Bacteriol. 1991 Jul;173(13):3966-76 - PubMed
    1. Science. 1990 Jan 5;247(4938):72-4 - PubMed
    1. Nature. 1991 Oct 31;353(6347):815-21 - PubMed
    1. J Bacteriol. 1990 Dec;172(12):6783-8 - PubMed