Gene expression profiles of glucose toxicity-exposed islet microvascular endothelial cells

Abstract

Objective: Islet microcirculation is mainly composed by IMECs. The aim of the study was to investigate the differences in gene expression profiles of IMECs upon glucose toxicity exposure and insulin treatment.

Methods: IMECs were treated with 5.6 mmol L^-1 glucose, 35 mmol L^-1 glucose, and 35 mmol L^-1 glucose plus 10^-8 mol L^-1 insulin, respectively. Gene expression profiles were determined by microarray and verified by qPCR. GO terms and KEGG analysis were performed to assess the potential roles of differentially expressed genes. The interaction and expression tendency of differentially expressed genes were analyzed by Path-Net algorithm.

Results: Compared with glucose toxicity-exposed IMECs, 1574 mRNAs in control group and 2870 mRNAs in insulin-treated IMECs were identified with differential expression, respectively. GO and KEGG pathway analysis revealed that these genes conferred roles in regulation of apoptosis, proliferation, migration, adhesion, and metabolic process etc. Additionally, MAPK signaling pathway and apoptosis were the dominant nodes in Path-Net. IMECs survival and function pathways were significantly changed, and the expression tendency of genes from euglycemia and glucose toxicity exposure to insulin treatment was revealed and enriched in 7 patterns.

Conclusions: Our study provides a microcirculatory framework for gene expression profiles of glucose toxicity-exposed IMECs.

Keywords: diabetes; gene expression profile; glucose toxicity; islet microvascular endothelial cell; microcirculation.