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. 2006 Sep 29;313(5795):1935-42.
doi: 10.1126/science.1131127. Epub 2006 Sep 7.

Structure of the 70S ribosome complexed with mRNA and tRNA

Maria Selmer  1 , Christine M Dunham, Frank V Murphy 4th, Albert Weixlbaumer, Sabine Petry, Ann C Kelley, John R Weir, V Ramakrishnan
Affiliations

Structure of the 70S ribosome complexed with mRNA and tRNA

Maria Selmer et al. Science. .

Abstract

The crystal structure of the bacterial 70S ribosome refined to 2.8 angstrom resolution reveals atomic details of its interactions with messenger RNA (mRNA) and transfer RNA (tRNA). A metal ion stabilizes a kink in the mRNA that demarcates the boundary between A and P sites, which is potentially important to prevent slippage of mRNA. Metal ions also stabilize the intersubunit interface. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.

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