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Structures of Cas9 endonucleases reveal RNA-mediated conformational activation.
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA. Jinek M, et al. Science. 2014 Mar 14;343(6176):1247997. doi: 10.1126/science.1247997. Epub 2014 Feb 6. Science. 2014. PMID: 24505130 Free PMC article.
Sumoylation of Cas9 at lysine 848 regulates protein stability and DNA binding.
Ergünay T, Ayhan Ö, Celen AB, Georgiadou P, Pekbilir E, Abaci YT, Yesildag D, Rettel M, Sobhiafshar U, Ogmen A, Emre NT, Sahin U. Ergünay T, et al. Life Sci Alliance. 2022 Jan 12;5(4):e202101078. doi: 10.26508/lsa.202101078. Print 2022 Apr. Life Sci Alliance. 2022. PMID: 35022246 Free PMC article.