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. 1991 Jun 15;102(1):39-44.
doi: 10.1016/0378-1119(91)90535-j.

Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase

B A Arthington  1 , L G Bennett, P L Skatrud, C J Guynn, R J Barbuch, C E Ulbright, M Bard
Affiliations

Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase

B A Arthington et al. Gene. .

Abstract

The ERG3 gene from Saccharomyces cerevisiae has been cloned by complementation of an erg3-2 mutation. ERG3 is the putative gene encoding the C-5 sterol desaturase required for ergosterol biosynthesis. The functional gene has been localized on a 2.5-kb HindIII-BamHI fragment containing an open reading frame comprising 365 amino acids. Gene disruption resulting from a deletion/substitution demonstrates that ERG3 is not essential for cell viability or the sparking function.

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