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. 1991 Oct;17(4):609-14.
doi: 10.1007/BF00037047.

Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana

Q Lin  1 , Z C Chen, J F Antoniw, R F White
Affiliations

Isolation and characterization of a cDNA clone encoding the anti-viral protein from Phytolacca americana

Q Lin et al. Plant Mol Biol. 1991 Oct.

Abstract

Phytolacca anti-viral protein (PAP) was purified from Phytolacca leaves and the N-terminal was sequenced. A cDNA library was made from mRNAs isolated from Phytolacca leaves and cDNA clones for PAP were identified using oligonucleotide probes derived from the N-terminal amino acid sequence. The PAP-cDNA clone was sequenced from both directions. The predicted amino acid sequence of PAP was compared with the amino acid sequences of other ribosome-inactivating proteins. The identities of these proteins to PAP ranged from 29 to 38%, and a region was found in each with a sequence similar to the PAP sequence (AIQMVSEAARFKYI). Southern blot analysis indicates that PAP is encoded by a multi-gene family.

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