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. 1991 Nov 15;107(2):329-33.
doi: 10.1016/0378-1119(91)90335-9.

Cloning and sequencing a cDNA encoding human ribosomal protein S25

M L Li  1 , C Latoud, M S Center
Affiliations

Cloning and sequencing a cDNA encoding human ribosomal protein S25

M L Li et al. Gene. .

Abstract

A full-length cDNA clone has been isolated from a cDNA library prepared from mRNA of adriamycin-resistant human leukemia HL60 cells. The nucleotide sequence of this cDNA has been determined and the protein coded for by the gene identified. The cDNA encodes a polypeptide of 125 amino acids (aa) with a deduced Mr of 13750. The deduced aa sequence of this protein has 56% homology to yeast ribosomal protein S31. Western-blot analysis using antibodies directed against a synthetic peptide based on the deduced aa sequence identifies the gene product as the human ribosomal protein S25.

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