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Comparative Study
. 1996 Apr;34(4):953-8.
doi: 10.1128/jcm.34.4.953-958.1996.

Sequencing emm-specific PCR products for routine and accurate typing of group A streptococci

B Beall  1 , R Facklam, T Thompson
Affiliations
Comparative Study

Sequencing emm-specific PCR products for routine and accurate typing of group A streptococci

B Beall et al. J Clin Microbiol. 1996 Apr.

Abstract

Rapid sequence analysis of specific PCR products was used to accurately deduce emm types corresponding to the majority of the known group A streptococcal (GAS) M serotypes. The study involved 95 M type reference GAS strains and a survey of 74 recent clinical isolates. A high percentage of agreement between M type serology and the previously published 5' sequences of the emm genes of M type reference strains was noted. The 5' sequences for six established M protein genes--the emm-32, emm-34, emm-38, emm-40, emm-42, and emm-71 genes--were determined to supplement the existing emm sequence database. Rapid sequence analysis differentiated serologically M-nontypeable strains and was used to establish the probable.

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