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Page 1
Showing results for vitro mt
Search for Vintro MT instead (1 results)
A fluorescence-based imaging method to measure in vitro and in vivo mitophagy using mt-Keima.
Sun N, Malide D, Liu J, Rovira II, Combs CA, Finkel T. Sun N, et al. Nat Protoc. 2017 Aug;12(8):1576-1587. doi: 10.1038/nprot.2017.060. Epub 2017 Jul 13. Nat Protoc. 2017. PMID: 28703790
This protocol also describes how to use confocal microscopy to visualize mitophagy in living tissues obtained from mt-Keima transgenic mice. With this protocol, the mt-Keima probe can reliably be imaged within the first 60 min after tissue collection. ...Typically, …
This protocol also describes how to use confocal microscopy to visualize mitophagy in living tissues obtained from mt-Keima transgeni …
Melatonin-stimulated MSC-derived exosomes improve diabetic wound healing through regulating macrophage M1 and M2 polarization by targeting the PTEN/AKT pathway.
Liu W, Yu M, Xie D, Wang L, Ye C, Zhu Q, Liu F, Yang L. Liu W, et al. Stem Cell Res Ther. 2020 Jun 29;11(1):259. doi: 10.1186/s13287-020-01756-x. Stem Cell Res Ther. 2020. PMID: 32600435 Free PMC article.
In this study, we investigated whether melatonin (MT)-pretreated MSCs-derived exosomes (MT-Exo) could exert superior effects on diabetic wound healing, and we attempted to elucidate the underlying mechanism. METHODS: For the evaluation of the anti-inflammator …
In this study, we investigated whether melatonin (MT)-pretreated MSCs-derived exosomes (MT-Exo) could exert superior effects o …
Polymorphisms in the MT-ATP6 and MT-CYB genes in in vitro fertilization failure.
Mao GH, Wang YN, Xu M, Wang WL, Tan L, Tao SB. Mao GH, et al. Mitochondrial DNA. 2015 Feb;26(1):20-4. doi: 10.3109/19401736.2013.840612. Epub 2013 Oct 9. Mitochondrial DNA. 2015. PMID: 24102627
The purpose of this study was to investigate whether fertilization failure after in vitro fertilization could be explained by polymorphisms in MT-ATP6 and MT-CYB genes. We performed a prospective comparative study of 111 fresh IVF cycles in Han Chinese betwee …
The purpose of this study was to investigate whether fertilization failure after in vitro fertilization could be explained by polymor …
Chemical synthesis, characterisation and in vitro and in vivo metabolism of the synthetic opioid MT-45 and its newly identified fluorinated analogue 2F-MT-45 with metabolite confirmation in urine samples from known drug users.
McKenzie C, Sutcliffe OB, Read KD, Scullion P, Epemolu O, Fletcher D, Helander A, Beck O, Rylski A, Antonides LH, Riley J, Smith SA, Nic Daeid N. McKenzie C, et al. Forensic Toxicol. 2018;36(2):359-374. doi: 10.1007/s11419-018-0413-1. Epub 2018 Apr 5. Forensic Toxicol. 2018. PMID: 29963206 Free PMC article.
MT-45, 2F-, 3F- and 4F-MT-45 were synthesised and reference analytical data were reported. ...The major MT-45 metabolites identified in human in vitro studies and in authenticated human urine were phase I metabolites and should be incorporated as analy …
MT-45, 2F-, 3F- and 4F-MT-45 were synthesised and reference analytical data were reported. ...The major MT-45 metabolit …
Senescent Hepatocytes in Decompensated Liver Show Reduced UPR(MT) and Its Key Player, CLPP, Attenuates Senescence In Vitro.
Sen B, Rastogi A, Nath R, Shasthry SM, Pamecha V, Pandey S, Gupta KJ, Sarin SK, Trehanpati N, Ramakrishna G. Sen B, et al. Cell Mol Gastroenterol Hepatol. 2019;8(1):73-94. doi: 10.1016/j.jcmgh.2019.03.001. Epub 2019 Mar 13. Cell Mol Gastroenterol Hepatol. 2019. PMID: 30878663 Free PMC article.
Aim of this study was to identify hepatocyte senescence and the role of UPR(MT) in cryptogenic cirrhosis. METHODS: Doxorubicin was used to induce senescence in non-neoplastic hepatocytes (PH5CH8) and hepatoma cells (HepG2 and Huh7). ...Dysfunctional mitochondria and …
Aim of this study was to identify hepatocyte senescence and the role of UPR(MT) in cryptogenic cirrhosis. METHODS: Doxorubicin …
Lead(II) Binding in Metallothioneins.
Wong DL, Merrifield-MacRae ME, Stillman MJ. Wong DL, et al. Met Ions Life Sci. 2017 Apr 10;17:/books/9783110434330/9783110434330-009/9783110434330-009.xml. doi: 10.1515/9783110434330-009. Met Ions Life Sci. 2017. PMID: 28731302 Free article. Review.
In vitro, metallation with a wide range of metals (especially mercury, arsenic, and lead) has been reported using a variety of analytical methods. To fully understand MT and its role with lead metabolism, we will describe how MT interacts with a wide v …
In vitro, metallation with a wide range of metals (especially mercury, arsenic, and lead) has been reported using a variety of analyt …
Identification of MT-45 Metabolites: In Silico Prediction, In Vitro Incubation with Rat Hepatocytes and In Vivo Confirmation.
Montesano C, Vannutelli G, Fanti F, Vincenti F, Gregori A, Rita Togna A, Canazza I, Marti M, Sergi M. Montesano C, et al. J Anal Toxicol. 2017 Oct 1;41(8):688-697. doi: 10.1093/jat/bkx058. J Anal Toxicol. 2017. PMID: 28985323
The detected metabolites were firstly predicted in silico, then incubation of the drug with rat hepatocytes was carried out and the obtained metabolites were identified by LC-HRMS, with retention times, mass shift between theoretical mass and observed mass (<5 ppm), peak abund …
The detected metabolites were firstly predicted in silico, then incubation of the drug with rat hepatocytes was carried out and the obtained …
In vitro-in vivo correlations (IVIVCs) of deposition for drugs given by oral inhalation.
Newman SP, Chan HK. Newman SP, et al. Adv Drug Deliv Rev. 2020 Dec;167:135-147. doi: 10.1016/j.addr.2020.06.023. Epub 2020 Jun 25. Adv Drug Deliv Rev. 2020. PMID: 32593641 Review.
MTs that mimic the human anatomy, coupled with simulated breathing patterns, have yielded estimates of lung dose from in vitro data that closely match those from in vivo gamma scintigraphic or pharmacokinetic studies. However, different models of MTs do not always yield id …
MTs that mimic the human anatomy, coupled with simulated breathing patterns, have yielded estimates of lung dose from in vitro data t …
Visualizing kinetochore architecture.
Alushin G, Nogales E. Alushin G, et al. Curr Opin Struct Biol. 2011 Oct;21(5):661-9. doi: 10.1016/j.sbi.2011.07.009. Epub 2011 Aug 22. Curr Opin Struct Biol. 2011. PMID: 21862320 Free PMC article. Review.
Here we review recent advances in the study of core MT-binding kinetochore complexes using electron microcopy methods in vitro and nanometer-accuracy fluorescence microscopy in vivo. ...There is a growing consensus that kinetochores are highly dynamic, supra- …
Here we review recent advances in the study of core MT-binding kinetochore complexes using electron microcopy methods in vi …
Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort-TE magnetization transfer (UTE-MT) imaging.
Guo T, Song Y, Tong J, Jiao S, Shen C, Wang H, Cui J, Dai D, Ma J, Chen M. Guo T, et al. Magn Reson Med. 2024 Oct;92(4):1658-1669. doi: 10.1002/mrm.30144. Epub 2024 May 9. Magn Reson Med. 2024. PMID: 38725197
PURPOSE: This study aims to assess ultrashort-TE magnetization transfer (UTE-MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy. ...CONCLUSION: This study highlighted the potential of UTE-MT parameters for evaluation of s …
PURPOSE: This study aims to assess ultrashort-TE magnetization transfer (UTE-MT) imaging of collagen degradation using an in vitro …
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