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. 1988 Dec 19;242(1):36-40.
doi: 10.1016/0014-5793(88)80980-3.

Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli

S Aoyama  1 , N Yoshida, S Inouye
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Free article

Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli

S Aoyama et al. FEBS Lett. .
Free article

Abstract

The lipase gene from Pseudomonas fragi IFO-12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein of Mr 29,966, which was close to the value of the lipase expressed in E. coli.

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