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. 1989 Sep 11;255(1):63-6.
doi: 10.1016/0014-5793(89)81061-0.

Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3

M Kriechbaum  1 , H J Heilmann, F J Wientjes, M Hahn, K D Jany, H G Gassen, F Sharif, G Alaeddinoglu
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Free article

Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3

M Kriechbaum et al. FEBS Lett. .
Free article

Abstract

A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12,000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5'- and a longer 3'-noncoding region. The deduced protein sequence was verified by partial peptide sequencing.

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