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. 1991 Nov 11;1090(3):348-50.
doi: 10.1016/0167-4781(91)90201-v.

Nucleotide sequence of trpE, anthranilate synthase I gene, of Bacillus caldotenax

A Shiratsuchi  1 , S Sato
Affiliations

Nucleotide sequence of trpE, anthranilate synthase I gene, of Bacillus caldotenax

A Shiratsuchi et al. Biochim Biophys Acta. .

Abstract

trpE gene of Bacillus caldotenax was cloned by complementation of an Escherichia coli tryptophan auxotroph. The trpE encoding a 508 amino acid polypeptide is followed by trpD. S1-nuclease mapping of a trpE in vivo transcript suggested that a promoter preceding the trpE was utilized by E. coli. The deduced amino acid sequence of anthranilate synthase I (ASI) was compared with that of five other bacterials ASIs.

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