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. 1992 Apr 1;89(7):2624-8.
doi: 10.1073/pnas.89.7.2624.

Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene

D S Knutzon  1 , G A Thompson, S E Radke, W B Johnson, V C Knauf, J C Kridl
Affiliations

Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene

D S Knutzon et al. Proc Natl Acad Sci U S A. .

Abstract

Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing rapeseed embryos during storage lipid biosynthesis. The resulting transgenic plants showed dramatically increased stearate levels in the seeds. A continuous distribution of stearate levels from 2% to 40% was observed in seeds of a transgenic B. napus plant, illustrating the potential to engineer specialized seed oil compositions.

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