Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Nov;22(5):345-55.
doi: 10.1007/BF00352435.

Complementation of Saccharomyces cerevisiae acid phosphatase mutation by a genomic sequence from the yeast Yarrowia lipolytica identifies a new phosphatase

B Y Tréton  1 , M T Le Dall, C M Gaillardin
Affiliations

Complementation of Saccharomyces cerevisiae acid phosphatase mutation by a genomic sequence from the yeast Yarrowia lipolytica identifies a new phosphatase

B Y Tréton et al. Curr Genet. 1992 Nov.

Abstract

A Yarrowia lipolytica gene library was constructed in vector YRp7 and transformed into a Saccharomyces cerevisiae strain lacking both major acid phosphatase activities. A 2.18 kb genomic sequence restoring the ability to hydrolyze alpha-naphthyl phosphate was isolated. Its sequencing revealed an ORF encoding 358 amino acids without significant homology with any known phosphatase. A putative signal peptide and several possible sites for N-glycosylation were identified. Phosphate-regulated expression of the cloned gene was observed in Y. lipolytica. Disruption data favoured the hypothesis that it might encode a minor phosphatase species.

PubMed Disclaimer

References

    1. J Bacteriol. 1987 Oct;169(10):4621-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2157-61 - PubMed
    1. Gene. 1987;57(2-3):267-72 - PubMed
    1. Curr Genet. 1987;11(5):377-83 - PubMed

Publication types

LinkOut - more resources