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. 1993 Apr;36(4):552-60.
doi: 10.1002/art.1780360417.

Nucleosome-specific antibody from an autoimmune MRL/Mp-lpr/lpr mouse

J A Losman  1 , T M Fasy, K E Novick, M Massa, M Monestier
Affiliations

Nucleosome-specific antibody from an autoimmune MRL/Mp-lpr/lpr mouse

J A Losman et al. Arthritis Rheum. 1993 Apr.

Abstract

Objective: To characterize the binding properties and variable-region sequences of LG4-1, a monoclonal antibody from an autoimmune MRL/Mp-lpr/lpr mouse that reacts specifically with nucleosome core particles and represents a new antinuclear antibody specificity.

Methods: The reactivity of the antibody against various nuclear substrates was determined using an enzymatic immunoassay, and the variable-region genes were sequenced from messenger RNA, using the dideoxy chain termination method.

Results: LG4-1 was found to react with nucleosome core particles but not with individual histones and DNA, or with various histone-histone and histone-DNA complexes. It was demonstrated that this antibody is encoded by a combination of variable-region genes and gene segments that have undergone few somatic mutations.

Conclusion: The nucleosome core particle expresses a unique conformational autoepitope(s) resulting from the ordered association of histones and DNA.

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