Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Dec;174(24):8158-62.
doi: 10.1128/jb.174.24.8158-8162.1992.

Analysis of operons encoding 23S rRNA of Clostridium botulinum type A

A K East  1 , D E Thompson, M D Collins
Affiliations

Analysis of operons encoding 23S rRNA of Clostridium botulinum type A

A K East et al. J Bacteriol. 1992 Dec.

Abstract

Southern hybridization analysis of Clostridium botulinum type A chromosomal DNA indicated the presence of six copies of the 23S rRNA gene. Fragments of DNA encoding 23S rRNA were amplified by polymerase chain reaction and cloned in Escherichia coli. Three clones examined by restriction enzyme and sequence analysis were found to be derived from different operons. Sequence determination of the entire insert of two clones revealed nine nucleotide changes in the genes coding for 23S rRNA (99.7% sequence identity) between operons encoded on the same chromosome, showing microheterogeneity in the rRNA operons of this organism.

PubMed Disclaimer

References

    1. Mol Gen Genet. 1977 Nov 14;156(2):185-93 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. FEMS Microbiol Lett. 1992 Jul 1;73(1-2):75-80 - PubMed
    1. FEMS Microbiol Lett. 1992 Sep 15;75(2-3):219-24 - PubMed
    1. J Clin Microbiol. 1991 Nov;29(11):2618-20 - PubMed

Publication types