Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1994 Dec;176(23):7387-90.
doi: 10.1128/jb.176.23.7387-7390.1994.

Characterization of the Thermus thermophilus locus encoding peptide deformylase and methionyl-tRNA(fMet) formyltransferase

T Meinnel  1 , S Blanquet
Affiliations
Comparative Study

Characterization of the Thermus thermophilus locus encoding peptide deformylase and methionyl-tRNA(fMet) formyltransferase

T Meinnel et al. J Bacteriol. 1994 Dec.

Abstract

An Escherichia coli strain with thermosensitive expression of the gene encoding peptide deformylase (fms) has been constructed. At nonpermissive temperatures, this strain fails to grow. The essential character of the fms gene was further used to clone by heterologous complementation the locus corresponding to Thermus thermophilus peptide deformylase. The cloned fragment also carries the methionyl-tRNA(fMet) formyltransferase gene (fmt). It is located immediately downstream from the fms gene, as in E. coli. Further sequence analysis of the region surrounding the E. coli fms-fmt locus indicates that the genes bordering the fms-fmt region are not conserved in T. thermophilus.

PubMed Disclaimer

References

    1. J Bacteriol. 1980 Jul;143(1):529-30 - PubMed
    1. Biochemistry. 1969 Jan;8(1):435-43 - PubMed
    1. Nucleic Acids Res. 1985 Jun 11;13(11):3891-903 - PubMed
    1. Cell. 1987 Jul 31;50(3):495-508 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 - PubMed

Publication types

Associated data

LinkOut - more resources